Unlike conventional treatments that damage healthy tissues upon dose escalation, ADCs utilize monoclonal antibodies (mAbs) to specifically bind tumour-associated target antigens and deliver a highly potent cytotoxic agent. chemotherapeutics, via a stable linker, has given rise to an extremely efficacious class of anti-cancer drugs with an already large and rapidly growing clinical pipeline. The primary objective of this paper is to review current knowledge and latest developments in the field of ADCs. Upon intravenous administration, ADCs bind to their target antigens and are internalized through receptor-mediated endocytosis. This facilitates the subsequent release of the cytotoxin, which eventually 2-NBDG leads to apoptotic cell death of the cancer cell. The three components of ADCs (mAb, linker and cytotoxin) affect the efficacy and toxicity of the conjugate. Optimizing each one, while enhancing the functionality of the ADC as a whole, has been one of the major considerations of ADC design and development. In addition to these, the choice of clinically relevant targets and the position and number of linkages have also been the key determinants of ADC efficacy. The 2-NBDG only marketed ADCs, brentuximab vedotin and trastuzumab emtansine (T-DM1), have demonstrated their use against both haematological and solid malignancies respectively. The success of future ADCs relies on improving target selection, increasing cytotoxin potency, developing innovative linkers and overcoming drug resistance. As more research is conducted to tackle these issues, ADCs are likely to become part of the future of targeted cancer therapeutics. == INTRODUCTION == The advent of modern-day cancer chemotherapy dates back to the mid-1900s when a chemical warfare agent known as nitrogen mustard was seen to destroy the bone marrow and lymph tissue of exposed individuals [1]. In the following years, nitrogen mustard, along with numerous other alkylating agents [2] took centre stage in the treatment of various haematological malignancies including leukaemia, lymphoma, Hodgkin’s disease and multiple myeloma. Several other serendipitous observations [3] lead to the development of the first primitive classes of cytotoxins (Figure 1). Despite vast progress in the field of cancer chemotherapy, small-molecule cancer drugs (although highly potent) continue to be plagued with the problems of non-specific toxicity (as a result of targeting all rapidly dividing cells), narrow therapeutic windows [4] and increasing resistance rates [5]. These concerns emphasize the need to 2-NBDG move away from conventional cancer treatments and explore new ways to tackle the ever-present disease. == Figure 1. Evolution of chemotherapeutic drugs [6]. == In recent years, enhanced understanding of cancer biology has shifted the focus of cancer treatment from traditional chemotherapy to targeted cancer therapies that take advantage of the differentiating features of tumour cells to provide a framework for drug development. These distinctive features, collectively known as the hallmarks of cancer [7,8] enable tumour cells to survive, multiply and metastasize using a variety of mechanisms including activation of self-sufficient growth signals, evasion of anti-growth signals, evasion of apoptosis and induction EIF4G1 of angiogenesis. Currently approved targeted therapies counteract these and provide safer and more efficacious alternatives to traditional chemotherapy. Cancer cells differ from normal cells due to genomic mutations in oncogenes and/or tumour suppressor genes [9]. Once the integrity of the genome is compromised, cells are more likely to develop additional genetic faults, some of which may give rise to tumour-specific antigens (found only on the surface of tumour cells) or tumour-associated antigens (overexpressed on tumour cells, but also present on normal cells) [10]. Ongoing research has found that several human cancers express unique tumour-specific or tumour-associated cell surface antigens [11] which are of great value as targets for large molecule, monoclonal antibody (mAb)-based therapy. The use of antibodies as magic bullets to treat disease was first proposed more than 100 years ago by the founder of chemotherapy, Paul Ehrlich [12]. Due to several challenges in the development of human antibodies, it was only in 1997 that the US FDA (Food and Drug Administration) approved the first anti-cancer antibody, rituximab, for the treatment of B-cell non-Hodgkin’s lymphoma [13]. Early mAbs were based on murine or chimeric antibodies that were modified to target human antigens. As these were non-human antibodies, they evoked a strong immune response that prevented the treatment from being successful. The large size of the mAbs also proved to be problematic as it resulted in reduced tumour penetration [14] and poor therapeutic effect. Since then, several advances in antibody engineering [15,16] have optimized pharmacokinetics and effector function while reducing immunogenicity. This 2-NBDG has resulted in a significant increase in the development of antibody-based drugs [17,18] against cancer. mAbs exert their therapeutic effect [19] by binding to tumour-specific or tumour-associated cell surface antigens. Once bound, the mAb kills the tumour cell by one or more of the following mechanisms; (i) abrogation of tumour cell signalling, resulting in apoptosis (ii) modulation of T-cell function through antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) or complement-dependent cell-mediated cytotoxicity (CDCC) and (iii) exertion of inhibitory effects on tumour vasculature and stroma [20,21]. Despite these.