B16F10 melanoma cells were bought from American Type Tradition Collection (Manassas, VA, USA). Flow cytometry Cells were stained for 15?min in 4C with antibodies against mouse Compact disc3, Compact disc69,
Furthermore, a prophylactic vaccination regimen comprising primary vaccination of na?ve cattle followed by two booster vaccinations 1.5 and PHT-427 6 months later could potentially maintain herd immunity over a period